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53 gel shift binding buffer  (Promega)

 
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    Promega 53 gel shift binding buffer
    53 Gel Shift Binding Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/53+gel+shift+binding+buffer/53+gel+shift+binding+buffer/pm11313369-107-27-31
    Average 90 stars, based on 1 article reviews
    53 gel shift binding buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: The role of NF-kappa B in TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis of melanoma cells.
    Article Snippet: .. The nuclear pellet then was resuspended in 30 ml of Dignam buffer C. A total of 10 mgrams of supernatant was incubated with 4 ml of 53 gel shift binding buffer (Promega, Madison, WI) for 30 min at room temperature to double-stranded oligonucleotides encoding the consensus binding sites of NF-kB (59-AGTTGAGGGGACTT TCCCAGGC-39) that had been labeled with [32P]ATP (GeneWorks, Adelaide, Australia) by polynucleotide kinase (Promega). ..

    Gel Shift:

    Article Title: The role of NF-kappa B in TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis of melanoma cells.
    Article Snippet: .. The nuclear pellet then was resuspended in 30 ml of Dignam buffer C. A total of 10 mgrams of supernatant was incubated with 4 ml of 53 gel shift binding buffer (Promega, Madison, WI) for 30 min at room temperature to double-stranded oligonucleotides encoding the consensus binding sites of NF-kB (59-AGTTGAGGGGACTT TCCCAGGC-39) that had been labeled with [32P]ATP (GeneWorks, Adelaide, Australia) by polynucleotide kinase (Promega). ..

    Article Title: Leukoregulin upregulation of prostaglandin endoperoxide H synthase-2 expression in human orbital fibroblasts
    Article Snippet: .. Aliquots of nuclear extract (2 μg protein/sample) were preincubated with 2 μl of 53 gel shift binding buffer (Promega, Madison, WI) containing 20% glycerol, 5 mM MgCl2, 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris ·HCl, pH 7.5, and 0.25 mg/ml poly(dI-dC) ·poly(dI-dC) in a total reaction volume of 10 μl for 10 min. ..

    Binding Assay:

    Article Title: The role of NF-kappa B in TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis of melanoma cells.
    Article Snippet: .. The nuclear pellet then was resuspended in 30 ml of Dignam buffer C. A total of 10 mgrams of supernatant was incubated with 4 ml of 53 gel shift binding buffer (Promega, Madison, WI) for 30 min at room temperature to double-stranded oligonucleotides encoding the consensus binding sites of NF-kB (59-AGTTGAGGGGACTT TCCCAGGC-39) that had been labeled with [32P]ATP (GeneWorks, Adelaide, Australia) by polynucleotide kinase (Promega). ..

    Article Title: Leukoregulin upregulation of prostaglandin endoperoxide H synthase-2 expression in human orbital fibroblasts
    Article Snippet: .. Aliquots of nuclear extract (2 μg protein/sample) were preincubated with 2 μl of 53 gel shift binding buffer (Promega, Madison, WI) containing 20% glycerol, 5 mM MgCl2, 2.5 mM EDTA, 2.5 mM DTT, 250 mM NaCl, 50 mM Tris ·HCl, pH 7.5, and 0.25 mg/ml poly(dI-dC) ·poly(dI-dC) in a total reaction volume of 10 μl for 10 min. ..

    Labeling:

    Article Title: The role of NF-kappa B in TNF-related apoptosis-inducing ligand (TRAIL)-induced apoptosis of melanoma cells.
    Article Snippet: .. The nuclear pellet then was resuspended in 30 ml of Dignam buffer C. A total of 10 mgrams of supernatant was incubated with 4 ml of 53 gel shift binding buffer (Promega, Madison, WI) for 30 min at room temperature to double-stranded oligonucleotides encoding the consensus binding sites of NF-kB (59-AGTTGAGGGGACTT TCCCAGGC-39) that had been labeled with [32P]ATP (GeneWorks, Adelaide, Australia) by polynucleotide kinase (Promega). ..



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    53 gel shift binding buffer  (Promega)
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